🧬 Molecular Biology Protocols

Comprehensive Resource Hub for PCR Technologies

📌 Note: This resource hub provides links to high-quality, peer-reviewed protocols and educational materials for various PCR and sequencing technologies. Always follow your institution's specific safety guidelines and protocol requirements.

🔬 PCR (Polymerase Chain Reaction)

Standard PCR protocols for DNA amplification using thermostable DNA polymerase. Essential for molecular cloning, diagnostics, and research applications.

PCR Basic Protocol Plus Troubleshooting PMC PCR Setup & Component Considerations Thermo Fisher Polymerase Chain Reaction (PCR) Overview StatPearls Standard PCR Protocol Sigma-Aldrich PCR Protocol & Troubleshooting Guide Boster Bio

🧪 RT-PCR (Reverse Transcription PCR)

Protocols for converting RNA to cDNA followed by PCR amplification. Critical for gene expression analysis and RNA virus detection.

RT-PCR Guidelines (One-Step & Two-Step) QIAGEN Five Steps to Fast RT-PCR Thermo Fisher Beginner's Guide to RT-PCR & qPCR The Biochemist Basic Principles of RT-qPCR Thermo Fisher Reverse Transcription Setup Guide Thermo Fisher

♾️ LAMP (Loop-Mediated Isothermal Amplification)

Isothermal amplification method requiring no thermal cycler. Ideal for point-of-care diagnostics and field testing with rapid visual detection.

Loop-Mediated Isothermal Amplification Guide NEB LAMP Products & Protocols Thermo Fisher Loop-mediated Isothermal Amplification of DNA PMC LAMP with Visual Detection Protocol Nature Protocols LAMP: The Better Sibling of PCR? PMC

💧 Digital PCR (dPCR)

Absolute quantification method using sample partitioning and Poisson statistics. Superior for rare variant detection and precise quantification.

dPCR for Beginners - Complete Guide QIAGEN Fundamentals of Digital PCR QIAGEN dPCR Protocol & Methods Sigma-Aldrich Digital PCR Technology Overview Sigma-Aldrich dPCR Setup & Troubleshooting Guide QIAGEN

🧬 NGS (Next-Generation Sequencing)

High-throughput sequencing protocols for whole genome, exome, and targeted sequencing. Includes library preparation and data analysis guidelines.

NGS Illumina Workflow - 4 Key Steps Thermo Fisher NGS for Beginners - Complete Guide Illumina NGS Workflow Steps & Best Practices Illumina NGS Experimental Design & Guidance Illumina NGS Validation Guidelines (AMP/CAP) PMC NGS Technology: Current Trends & Advancements PMC

✂️ CRISPR-PCR Technologies

CRISPR-Cas9 based detection methods combining gene editing specificity with PCR amplification for highly specific DNA/RNA detection and typing.

CRISPR-typing PCR (ctPCR) Protocol Nature One-Pot CRISPR/Cas9-Typing PCR Method ScienceDirect Validating CRISPR/Cas9 Gene Editing Sigma-Aldrich Comprehensive CRISPR Guide Addgene CRISPR 101: Validating Genome Edits Addgene CRISPR-Cas Pathogen Detection Methods Frontiers